Biotium introduces unique super-sensitive RNA prestain loading dye

January 5, 2022

EMBER500™ RNA Prestain Loading Dye allows single-step denaturing, loading, and bright RNA staining on regular agarose gels

EMBER500™ RNA Prestain Loading Dye Biotium, inventor of the popular GelRed® and GelGreen® DNA gel stains, announces the release of EMBER500™ RNA Prestain Loading Dye. This novel prestain loading dye is much more sensitive than conventional prestaining with ethidium bromide (EtBr). In addition, it is compatible with blue LED gel imagers, unlike EtBr.

Staining for RNA is commonly done with EtBr on a denaturing gel after electrophoresis. However, denaturing gels can be complicated to prepare and involve handling hazardous reagents. RNA prestaining with EtBr can be performed with non-denaturing agarose gels, but requires large amounts of the dye and sensitivity is still severely limited. EMBER500™ was developed by Biotium scientists to offer much brighter signal and higher sensitivity than EtBr for RNA gel staining. For maximum convenience, the prestain also includes formamide as well as electrophoresis tracking dyes, allowing sample denaturing, loading, tracking, and staining in a single step. In addition, EMBER500™ stains both RNA and DNA, allowing the detection of contaminating genomic DNA in purified RNA samples. Detection of EMBER500™ is flexible, with compatibility for UV transilluminators and blue LED gel imagers, which eliminate UV exposure hazards.

The prestain will be supplied at a 2X concentration, for convenient loading of sample and dye in equal volumes. EMBER500™ RNA Prestain Loading Dye will be available in 4 x 1 mL and 1 x 250 uL sizes priced at $350 and $60 respectively. However, free samples will be available for a limited time. Please visit the product page to learn more about EMBER500™ and how to receive a free sample.

Features and benefits of EMBER500™ RNA Prestain Loading Dye

  • Sensitive: Brighter and much more sensitive than EtBr
  • Convenient: Denature, load, track, and stain RNA samples in a single step on a regular agarose gel
  • Versatile: Stains both DNA and RNA for evaluating total RNA integrity and DNA contamination
  • Flexible Detection: Detect with UV transilluminators or blue LED gel imagers

The release of EMBER500™ RNA Prestain Loading Dye adds to Biotium’s industry-leading catalog of novel nucleic acid gel stains for life science research.

About Biotium

Biotium is a leading life science reagent manufacturer and supplier devoted to providing high-quality and innovative fluorescent tools that fuel scientific discovery. Our collaborative team of experienced chemists and biologists, who are at the forefront of fluorescent dye design, apply chemistry-based principles toward producing solutions for unmet challenges in life science and medical research. Since its founding in 2001, Biotium has developed over 30 patented technologies that have been licensed out to leading life science technology companies worldwide. For more information, please visit

Categories: Omics & Genetics, Biotechnology
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